崇仁麻鸡小肠PepT1 mRNA基因差异表达

doi:10.11843/j.issn.0366-6964.2014.02.004

Abstract

Abstract:

This experiment was conducted to observe the expression of PepT1 mRNA in Chongren Spotty chicken intestine in order to support the study on the mechanism of transporting small peptide through small intestine and the distribution of peptide transporters.Samples were obtained from intestine of 120-day-old Chongren Spotty chicken and real-time fluorescent quantitative PCR was used to study the differential expression of peptide transporter 1 (PepT1) mRNA in different intestinal segments and breeds with different feed conversion rate.The results showed that the abundance of PepT1 mRNA gradually decreased from duodenum to jejunum and ileum，the expression of PepT1 mRNA in duodenum was significantly higher than that in ileum (P<0.01)，but there was no significant difference in the abundance of PepT1 mRNA between duodenum and jejunum or between jejunum and ileum (P>0.05).The abundance of PepT1 mRNA from breed with high feed conversion rate was higher than that from breed with low feed conversion rate (P>0.05).The results indicate that the anterior small intestine is the primary site for the expression of PepT1 mRNA and the abundance of PepT1 mRNA is the highest in the duodenum，meanwhile，Chongren Spotty chicken with high feed conversion rate has higher PepT1 mRNA abundance compared with those with low feed conversion rate.

CLC Number:

S831.2

XIAO Jin-hua，WU Yan-ping，HE Yu-yong，HUO Jun-hong，LIU Lin-xiu,JI Hua-yuan，XIE Ming-gui,KANG Zhao-feng，XIE Jin-fang，LU Wei. Differential Expression of PepT1 mRNA in Small Intestine of Chongren Spotty Chicken[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(2): 191-196.

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Differential Expression of PepT1 mRNA in Small Intestine of Chongren Spotty Chicken

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